Enzo Life Sciences的ROS-ID??Superoxide detection kit可利用熒光顯微鏡和/或流式細(xì)胞儀直接實(shí)時(shí)監(jiān)測(cè)活細(xì)胞中超氧化物的產(chǎn)生。該試劑盒的一個(gè)主要成分，超氧化物檢測(cè)試劑（橙色），是一種細(xì)胞滲透性探針，可與超氧化物發(fā)生特異性反應(yīng)，產(chǎn)生橙色熒光產(chǎn)物。熒光探針對(duì)活性過(guò)氧化物、羥基、過(guò)氧亞硝酸鹽、氯或溴相對(duì)不敏感，因此該試劑盒不能檢測(cè)這些分析物。染色后，可使用配備有標(biāo)準(zhǔn)橙色（如550/620 nm）濾光片的寬場(chǎng)顯微鏡進(jìn)行觀察，或使用配備有藍(lán)色（488 nm）激光的流式細(xì)胞儀進(jìn)行分析。
超氧化物檢測(cè)試劑盒包含足夠的試劑，可使用活細(xì)胞（貼壁或懸?。┻M(jìn)行至少200次顯微鏡分析或50次流式細(xì)胞術(shù)分析。
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產(chǎn)品特點(diǎn)
可通過(guò)熒光顯微鏡或流式細(xì)胞術(shù)直接監(jiān)測(cè)活細(xì)胞中超氧化物的整體水平
高靈敏度、特異性和準(zhǔn)確性，可用于活細(xì)胞研究
與組織培養(yǎng)基的主要成分（酚紅、FBS和BSA）兼容
試劑盒內(nèi)包括整套所需試劑，包括ROS誘導(dǎo)劑和清除劑
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ENZ-51012 |
產(chǎn)品名稱 | ROS-ID??Superoxide detection kit |
規(guī)格 | 1*1Kit |
短期保存 | -20°C |
長(zhǎng)期保存 | -80°C |
試劑盒組分 | Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 μmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack |
應(yīng)用 | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
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部分產(chǎn)品引用文獻(xiàn)
1.?Rational design of mitochondria targeted thiabendazole-based Ir (III) biscyclometalated complexes for a multimodal photodynamic therapy of cancer: I. Echevarria, et al.; J. Inorg. Biochem. 231, 111790 (2022)
2.?A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration: K. Bijnens, et al.; Biomolecules 11, 714 (2021)
3.?An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination: Y. Wang, et al.; Nat. Commun. 12, 4964 (2021)
4.?Assay for advanced glycation end products generating intracellular oxidative stress through binding to its receptor: T. Kobori, et al.; Anal. Biochem. 611, 114018 (2020)
5.?Epithelial cadherin regulates transition between the na?ve and primed pluripotent states in mouse embryonic stem cells: A.M. Sharaireh, et al.; Stem Cells 38, 1292 (2020)
6.?Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy: B. Montico, et al.; Cell Death Dis. 11, 392 (2020)
7.?PCSK9 expression in the ischemic heart and its relationship to infarct size, cardiac function and development of autophagy: Z. Ding, et al.; Cardiovasc. Res. 114, 1738 (2018)
8.?SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes: K.R. Aseer, et al.; Free Radic. Biol. Med. 108, 874 (2017)
9.?Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression: L. Pasterk, et al.; Sci. Rep. 6, 22104 (2016)
10.?TRPC6 channel activation promotes neonatal glomerular mesangial cell apoptosis via calcineurin/NFAT and FasL/Fas signaling pathways: H. Soni, et al.; Sci. Rep. 6, 29041 (2016)
11.?Hemodynamic Shear Stress via ROS Modulates PCSK9 Expression in Human Vascular Endothelial and Smooth Muscle Cells and Along the Mouse Aorta: Z. Ding, et al.; Antioxid. Redox Signal. 22, 760 (2015)
12.?Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells: N. Shin, et al.; Biochem. Biophys. Res. Commun. 459, 699 (2015)
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