Enzo Life Sciences的CYTO-ID?綠色/紅色長期細(xì)胞示蹤試劑盒采用專有的非共價(jià)細(xì)胞標(biāo)記技術(shù)，將含有疏水性脂肪鏈的熒光染料穩(wěn)定地結(jié)合入細(xì)胞膜的脂質(zhì)雙分子層中。標(biāo)記緩沖液對哺乳動(dòng)物細(xì)胞是等滲的，不含去污劑或有機(jī)溶劑。染料對細(xì)胞沒有毒性，可在細(xì)胞中保留長達(dá)96小時(shí)，并在有絲分裂時(shí)傳遞給子細(xì)胞。由于該染料不對細(xì)胞內(nèi)的蛋白質(zhì)進(jìn)行共價(jià)修飾，與基于硫醇反應(yīng)的氯甲基或胺反應(yīng)的琥珀酰亞胺酯類熒光染料的分子探針相比，細(xì)胞正常的生理反應(yīng)得到更好的保留。
也可以使用CELLESTIAL?染料進(jìn)行雙重標(biāo)記。標(biāo)記的細(xì)胞可以通過共聚焦熒光顯微鏡等儀器進(jìn)行觀察。此外，染料標(biāo)記的和未標(biāo)記的細(xì)胞群可通過流式細(xì)胞儀進(jìn)行分析。在延長96小時(shí)的培養(yǎng)后，沒有觀察到熒光轉(zhuǎn)移到相鄰細(xì)胞。這與Calcein AM和BCECF AM標(biāo)記形成鮮明對比，后者在生理溫度下只能在存活的細(xì)胞內(nèi)保留幾個(gè)小時(shí)。
Enzo Life Sciences的CYTO-ID?長期細(xì)胞示蹤試劑盒適用于多種應(yīng)用，如追蹤細(xì)胞譜系，長期細(xì)胞活力、細(xì)胞毒性、細(xì)胞粘附、細(xì)胞遷移和細(xì)胞-細(xì)胞融合研究。
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產(chǎn)品特點(diǎn)
● 可使用各種CELLESTIAL??熒光探針進(jìn)行雙重標(biāo)記
●?熒光從染料標(biāo)記的細(xì)胞轉(zhuǎn)移到未標(biāo)記的細(xì)胞的情況極少
●?適用于長期細(xì)胞存活率、細(xì)胞毒性、細(xì)胞粘附、細(xì)胞遷移和細(xì)胞間融合測定
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實(shí)驗(yàn)示例
圖1.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID??Green Tracer dye對Jurkat細(xì)胞的染色。使用標(biāo)準(zhǔn)的FITC（綠色）濾光片組對膜結(jié)合信號進(jìn)行成像。
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圖2.流式細(xì)胞術(shù)分析Jurkat細(xì)胞混合群隨時(shí)間變化的熒光。將用 CYTO-ID?綠色示蹤染料染色的Jurkat細(xì)胞與未染色的Jurkat細(xì)胞群混合并孵育120小時(shí)。
圖3.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID??Red Tracer dye對Jurkat細(xì)胞的染色。使用標(biāo)準(zhǔn)的Texas Red（紅色）濾光片組對膜結(jié)合信號進(jìn)行成像。
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圖4.熒光顯微鏡圖像顯示了用CYTO-ID??Red Tracer dye對HeLa細(xì)胞的染色。使用標(biāo)準(zhǔn)的Texas Red（紅色）濾光片組對膜結(jié)合信號進(jìn)行成像(20x)。
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圖5.流式細(xì)胞術(shù)分析Jurkat細(xì)胞混合群隨時(shí)間變化的熒光。將用 CYTO-ID?紅色示蹤染料染色的Jurkat細(xì)胞與未染色的Jurkat細(xì)胞群混合并孵育96小時(shí)。
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產(chǎn)品信息
產(chǎn)品貨號 | 產(chǎn)品名稱 | 規(guī)格 | 應(yīng)用 | 試劑盒組分 |
ENZ-51036-K025 | CYTO-ID?? Green long-term cell tracer kit | 1Kit | Flow Cytometry,? Fluorescence microscopy,? Fluorescent detection | CYTO-ID??Green tracer dye, 50μl
4X Labeling Buffer, 12.5ml
10X HBSS, 25ml |
ENZ-51037-K025 | CYTO-ID?? Red long-term cell tracer kit? | 1Kit | Flow Cytometry,? Fluorescence microscopy,? Fluorescent detection | CYTO-ID??Red Tracer Dye, 50 μl 4X Labeling Buffer, 12.5 ml 10X HBSS, 25 ml |
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部分產(chǎn)品引用文獻(xiàn)
1. Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model: R. An, et al.; FASEB J. 32, 5587 (2018), Application(s): Identification of TR-BME2 cells in tube-formation co-culture assay with CC521 colon adenocarcinoma cells
2. A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients: H. Zhang , et al.; Int. J. Oncol. 50, 1075 (2017), Application(s): Use with HOS cells (osteosarcoma). Microscopy
3.?INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responses: F. J. Sulzmaier, et al.; J. Immunother. Cancer 11, e006116 (2023)
4. Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer: R.R. Weng, et al.; Nat. Commun. 12, 2163 (2021)
5. Characterization and Cellular Internalization of Spherical Cellulose Nanocrystals (CNC) into Normal and Cancerous Fibroblasts: N.A.H. Shazali, et al.; Materials (Basel) 12, 3251 (2019), Application(s): C6 and NIH3T3 cell tracing
6. Time resolved 3D live-cell imaging on implants: A. Ingendoh-Tsakmakidis, et al.; PLoS One 13, e0205411 (2018)
7. Nerve guidance conduit with a hybrid structure of a PLGA microfibrousbundle wrapped in a micro/nanostructured membrane: S.W. Peng, et al.; Int. J. Nanomedicine 12, 421 (2017), Application(s):Tracking in vitro cells migration (immortalized neuron progenitor KT 98)
8. Thioreductase-containing epitopes inhibit the development of type 1 diabetes in the NOD mouse model: E. Malek Abrahimians, et al.; Front. Immunol. 7, 67 (2016), Application(s): Flow cytometry analysis using isolated mouse spleen B cells
9. Syngeneic murine ovarian cancer model reveals that ascites enriches for ovarian cancer stem-like cells expressing membrane GRP78: L. Mo, et al.; Mol. Cancer Ther. 14, 747 (2015)?
10. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture: Y. Takeshita, et al.; J. Neurosci. Methods 232, 165 (2014), Application(s): Confocal microscopy using adult human astrocyte cells
11. Vascular endothelial growth factor-C modulates proliferation and chemoresistance in acute myeloid leukemic cells through an endothelin-1-dependent induction of cyclooxygenase-2: K.T. Hua, et al.; Biochim. Biophys. Acta 1843, 387 (2014)
12. Expanding the Ig superfamily code for laminar specificity in retina: expression and role of contactins: M. Yamagata, et al.; J. Neurosci. 32, 14402 (2012)
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