瘦素(Leptin)是由ob基因編碼、在脂肪組織中生成的一種脂肪代謝調(diào)控產(chǎn)物，在代謝和調(diào)控體重等方面發(fā)揮重要作用。它通過(guò)下丘腦中的瘦素受體發(fā)出信號(hào)，降低食欲，增加能量消耗。在外周組織中，瘦素能拮抗胰島素信號(hào)傳導(dǎo)，增加脂肪酸氧化，減少胰島β細(xì)胞的胰島素分泌，并促進(jìn)生育。它在脂肪細(xì)胞中的表達(dá)可以被其他脂肪因子如IL-6（刺激）和TNF-α（抑制）以旁分泌方式調(diào)節(jié)。瘦素缺乏的小鼠表現(xiàn)出嚴(yán)重的胰島素抵抗、肥胖和生育能力下降，所有這些都可以通過(guò)外源性瘦素給藥而逆轉(zhuǎn)。
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●?無(wú)邊緣效應(yīng)：所有孵育步驟在常溫下進(jìn)行消除了37°C下蒸發(fā)的風(fēng)險(xiǎn)
●?檢測(cè)范圍廣：易于定量檢測(cè)高水平和低水平瘦素
●?方便：所有試劑保存在4°C
●?快速：僅需3小時(shí)就可獲得39個(gè)樣本的重復(fù)結(jié)果
●?預(yù)包被板和即用型試劑為您節(jié)省實(shí)驗(yàn)時(shí)間
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ADI-900-015A |
產(chǎn)品名稱 | Leptin (rat), ELISA kit（大鼠瘦素ELISA試劑盒） |
規(guī)格 | 96 wells |
靈敏度 | 67.2 pg/ml |
檢測(cè)范圍 | 100- 6,400 pg/ml |
檢測(cè)時(shí)間 | 3 hours |
樣本種屬 | Rat |
樣本類型 | culture supernatants, plasma, and serum |
保存溫度 | +4°C |
試劑盒組分 | Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate |
部分產(chǎn)品引用文獻(xiàn)
1. Altered motor, anxiety-related and attentional task performance at baseline associate with multiple gene copies of the vesicular acetylcholine transporter and related protein overexpression in ChAT::Cre+ rats: C.P. Mantanona, et al.; Brain Struct. Funct. 224, 3095 (2019)?
2. Long-term feeding of red algae (Gelidium amansii) ameliorates glucose and lipid metabolism in a high fructose diet-impaired glucose tolerance rat model: H.C. Liu, et al.; J. Food Drug Anal. 25, 543 (2017), Application(s): Determination of leptin, rat plasma?
3. Melatonin supplementation plus exercise behavior ameliorate insulin resistance, hypertension and fatigue in a rat model of type 2 diabetes mellitus: M.M. Rahman, et al.; Biomed. Pharmacother. 92, 606 (2017)?
4. Effect of Intermittent Hypoxia on Arcuate Nucleus in the Leptin-Deficient Rat: J. Ciriello, et al.; Neurosci. Lett. 626, 112 (2016), Application(s): Measured leptin levels in rat aqueous plasma phase?
5. Neonatal treatment with scopolamine butylbromide prevents metabolic dysfunction in male rats: A. Malta, et al.; Sci. Rep. 6, 30745 (2016), Application(s): Leptin level quantification?
6. Impact of prenatal exposure to obesogenic environment on later-life brown and white adipocytes and liver outcomes: L. Bezpalko, et al.; Proc. Shevchenko Sci. Soc. Medicine 41, 33 (2015), Application(s): ELISA using rat serum?
7. Lactobionic acid reduces body weight gain in diet-induced obese rats by targeted inhibition of galectin-1: R. Mukherjee, et al.; Biochem. Biophys. Res. Commun. 463, 1311 (2015), Application(s): ELISA using rat plasma
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