雌二醇(estradiol) 是由卵巢內(nèi)卵泡的顆粒細胞分泌的類固醇激素，是主要的雌激素，負責(zé)調(diào)節(jié)女性特征、附屬性器官的成熟和月經(jīng)-排卵周期，促進乳腺導(dǎo)管系統(tǒng)的產(chǎn)生，有α，β兩種類型。其代謝物是雌酮及雌三醇。雌二醇的靶器官為子宮、陰道、輸卵管和垂體。雌激素能促使細胞合成DNA、RNA和相應(yīng)組織內(nèi)各種不同的蛋白質(zhì)。β-雌二醇(17β-Estradiol)可引起促性腺激素的產(chǎn)生從而促進排卵。
ENZO 17β-Estradiol ELISA kit是一種比色競爭法酶免疫檢測試劑盒，3小時內(nèi)可以得到檢測結(jié)果。在405nm處讀取吸光度。
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●?特異性強，與相關(guān)類固醇交叉反應(yīng)性低
●?安全無放射性的酶法檢測
●?重復(fù)性好
●?簡單易用的顏色編碼試劑最大限度地減少錯誤
●?超越半定量Western Blot分析的全定量檢測
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產(chǎn)品信息
| 產(chǎn)品貨號 | ADI-900-008/ ADI-901-008 |
產(chǎn)品名稱 | 17β-Estradiol ELISA kit（17β-雌二醇ELISA試劑盒） |
規(guī)格 | 96 wells/?5x96 wells |
其他名稱 | Estrogen |
靈敏度 | 28.5 pg/ml |
檢測范圍 | 29.3-30,000 pg/ml |
檢測時間 | <3 hours |
樣本種屬 | Species independent |
樣本類型 | culture supernatants and saliva from any species. For serum and plasma use ADI-900-174 |
保存溫度 | +4°C |
試劑盒組分 | GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution n |
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部分產(chǎn)品引用文獻
1. Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022)?
2. A novel method to differentiate tonsil-derived mesenchymal stem cells in vitro into estrogen-secreting cells: H. Kim, et al.; Tissue Eng. Regen. Med. 18, 253 (2021), Application(s): ELISA using culture supernatants?
3. Chronic irisin exposure decreases sexual incentive motivation in female rats: N. Ulker, et al.; Physiol. Behav. 232, 113341 (2021), Application(s): Rat serum?
4. Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions: H.J. Yoon, et al.; Sci. Adv. 7, eabe8873 (2021)?
5. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats: L.C. Santos, et al.; Biol. Reprod. 61, 1093 (2021)?
6. Neonatal androgenization in rats affects oocyte maturation: G. Anesetti & R. Chávez-Genaro; Reprod Sci. 28, 2799 (2021)
7. Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021)?
8. Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants?
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