跟二甲苯 Say?‘‘Good Bye’’
? ? ? ? ——?新品封片劑?VectaMount? Express?Mounting Medium
?
→ 產(chǎn)品簡(jiǎn)介
VectaMount?Express Mounting Medium為非水溶性封片劑,可用于免疫組化實(shí)驗(yàn)中細(xì)胞和組織樣本的快速固定及封片。該產(chǎn)品采用獨(dú)特的配方,染色流程結(jié)束后,在異丙醇中簡(jiǎn)單清洗一下,即可進(jìn)行封片。
封片前無需繁瑣的乙醇脫水和溶劑清洗步驟,大大節(jié)省了實(shí)驗(yàn)時(shí)間。
→ 產(chǎn)品特點(diǎn)
● 無需大量的乙醇和其他溶劑的清洗;
●?可用于染色樣本的快速干燥及可視化觀察;
? ?室溫下保存18個(gè)月;
●?與辣根過氧化物酶HRP和堿性磷酸酶AP底物相兼容;
●?折射率為1.49
→ 產(chǎn)品使用
傳統(tǒng)染色-封片流程
使用VectaMount?Express染色-封片流程
?
→ 產(chǎn)品五大優(yōu)勢(shì)
| ?省時(shí):將15-30min的清洗操作縮短為2min; |
| ?經(jīng)濟(jì)節(jié)約:大大減少乙醇和二甲苯的花費(fèi)成本; |
| ?方便快捷:大大簡(jiǎn)化了封片流程,可直接封片觀察; |
| ?操作簡(jiǎn)便:即用型試劑,直接滴加即可; |
| ?無害、可持續(xù)操作:避免二甲苯對(duì)實(shí)驗(yàn)人員的傷害,無毒無害。 |
→ 與HRP/AP底物的兼容性
| H-5700 | H-5000 | H-5501 |
Substrate | Cat. No | VectaMount Express Mounting Medium (Non-Aqueous) | VectaMount Permanent Mounting Medium (Non-Aqueous) | VectaMount AQ Aqueous Mounting Medium |
Horseradish Peroxidase (HRP) |
| ImmPACT? DAB (brown) | SK-4105 | ? | ? | ? |
| ImmPACT DAB EqV (brown) | SK-4103 | ? | ? | ? |
| DAB (brown) | SK-4100 | ? | ? | ? |
| DAB-Ni (gray-black) | SK-4100 | ? | ? |
|
| ImmPACT AEC (red) | SK-4205 |
|
| ? |
| ImmPACT AMEC Red (red) | SK-4285 |
|
| ? |
| AEC (red) | SK-4200 |
|
| ? |
| TMB (blue) | SK-4400 |
| ? |
|
| ImmPACT VIP (purple) | SK-4605 | ? | ? |
|
| Vector? VIP (purple) | SK-4600 | ? | ? |
|
| ImmPACT SG (blue-gray) | SK-4705 | ? | ? | ? |
| Vector SG (blue-gray) | SK-4700 | ? | ? | ? |
| ImmPACT NovaRED? (red) | SK-4805 | ? | ? |
|
| Vector NovaRED (red) | SK-4800 | ? | ? |
|
Alkaline Phosphatase (AP) |
| ImmPACT Vector Red (magenta) | SK-5105 | ? | ? | ? |
| Vector Red (magenta) | SK-5100 | ? | ? | ? |
| Vector Black (brown-black) | SK-5200 |
| ? |
|
| Vector Blue (blue) | SK-5300 | ? | ? |
|
| BCIP/NBT (indigo) | SK-5400 | ? | ? | ? |
→ 產(chǎn)品應(yīng)用舉例
| |
上圖為人結(jié)腸組織染色結(jié)果:使用Mouse Anti-Ki67, ImmPRESS?-HRP Anti-Mouse IgG, ImmPACT??DAB EqV?以及?Rabbit Anti-Cytokeratin ImmPRESS-AP Anti-Rabbit IgG進(jìn)行雙染,封片劑為VectaMount Express Mounting | 上圖為人扁桃體組織染色結(jié)果:使用 Mouse Anti-Ki67, ImmPRESS-AP Anti-Mouse IgG,進(jìn)行單染,?Vector Blue顯色;?Nuclear Fast Red counterstain復(fù)染。.封片劑為VectaMount Express Mounting Media |
→ 產(chǎn)品訂購(gòu)信息
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