扁桃腺(石蠟切片),使用Antigen Unmasking Solution進(jìn)行抗原修復(fù),使用Anti-Pan Cytokeratin (mouse;?clone AE1/AE3),Biotinylated?Horse Anti-Mouse IgG和Cy3 Streptavidin進(jìn)行染色。使用VECTASHIELD HardSet Antifade Mounting Medium with DAPI進(jìn)行封片。
選擇Vectorlabs高品質(zhì)二抗-不再對二抗產(chǎn)生質(zhì)疑!
你是否曾經(jīng)對染色效果極差的實(shí)驗(yàn)效果圖感到失望?在您的實(shí)驗(yàn)過程中使用的二抗有可能影響染色質(zhì)量,因此選擇一個最高質(zhì)量的可靠的二抗對實(shí)驗(yàn)結(jié)果至關(guān)重要。
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是什么讓二抗更加可靠:
1.?正確的免疫方案,以確保對目標(biāo)抗原具有盡可能高的親和力。
2.?親和層析純化,以提高抗體的特異性。
3.?通過固相吸附去除交叉反應(yīng)性。
4.?達(dá)到不影響偶聯(lián)物穩(wěn)定性、抗體的特異性和親和力的最大偶聯(lián)度。
5.?執(zhí)行嚴(yán)格的質(zhì)量控制檢測。
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Vectorlabs二抗鏈接:
https://vectorlabs.com/browse/secondary-antibodies?utm_source=pardot&utm_medium=email&utm_campaign=secondaryab
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Vectorlabs最暢銷的二抗:
1.Amplified Fluorescent Staining Systems:
https://vectorlabs.com/browse/amplified-fluorescent-staining-systems?utm_source=pardot&utm_medium=email&utm_campaign=secondaryab
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2.Fluorophore-Conjugated Secondaries:
https://vectorlabs.com/browse/fluorophore-conjugated-secondaries?utm_source=pardot&utm_medium=email&utm_campaign=secondaryab
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3.Biotinylated Secondary Antibodies:
https://vectorlabs.com/products/antibodies/secondary-antibodies/biotinylated
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Vectorlabs其他二抗:
1.Enzyme-Conjugated Secondary Antibodies:
https://vectorlabs.com/products/antibodies/secondary-antibodies/enzyme-conjugated
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2.Unconjugated Secondary Antibodies:
https://vectorlabs.com/products/antibodies/secondary-antibodies/unconjugated
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更多詳情請聯(lián)系Vectorlabs中國授權(quán)獨(dú)家代理商欣博盛生物
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