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Soybean Agglutinin (SBA), Agarose bound, 2 ml/大豆凝集素，瓊脂糖交聯(lián)
品牌： Vectorlabs
產品貨號： AL-1013-2
規(guī)格： 2 ml

報價：
折后價：

數量： - +
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Soybean Agglutinin (SBA), Agarose bound, 2 ml/大豆凝集素，瓊脂糖交聯(lián)
  • 別名:
    大豆凝集素，瓊脂糖交聯(lián)
  • 產品描述:
    Soybean agglutinin (SBA) Agarose bound Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related isolectins. This glycoprotein has a molecular weight of about 120 kDa and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.An important application for SBA is the separation of pluripotent stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers.Agarose bound* Soybean agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine*4 mg lectin/ml gel
  • 反應種屬:
  • 宿主來源:
  • 實驗應用:
    Glycobiology,Affinity Chromatography
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  • 推薦稀釋度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M N-acetylgalactoseamine in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
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  • 偶聯(lián):
    Agarose
  • 產品濃度:
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  • 陽性對照:
  • 保存溫度:
    2° - 8°C – Do Not Freeze
  • 運輸溫度:
    Ambient
  • 細胞定位:
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  • 產品形式:
  • 存儲溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide, 0.01 mM MnCl2
  • 有效期:
  • 產地:
    美國
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  • 樣本類型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide, 0.01 mM MnCl2
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