Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.Fluorescein labeled Jacalin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose
反應(yīng)種屬:
宿主來(lái)源:
實(shí)驗(yàn)應(yīng)用:
Immunofluorescence,Glycobiology
靶標(biāo)/特異性:
同種型:
推薦稀釋度:
The recommended concentration range for use is 5-20μg/ml.
免疫原:
免疫原種屬:
克隆性:
克隆號(hào):
純化方式:
偶聯(lián):
Fluorescein
產(chǎn)品濃度:
5 mg active conjugate/ml
表達(dá)系統(tǒng):
陽(yáng)性對(duì)照:
保存溫度:
2° - 8°C – Do Not Freeze
運(yùn)輸溫度:
Ambient
細(xì)胞定位:
預(yù)期分子量:
產(chǎn)品形式:
存儲(chǔ)溶液:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide,
0.1 mM CaCl2, 10 mM lactose, 10 mM Beta-Cyclodextrin
有效期:
產(chǎn)地:
美國(guó)
外觀:
生產(chǎn)商:
功能與背景:
URL:
靈敏度:
檢測(cè)范圍:
檢測(cè)方法:
樣本類型:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide,
0.1 mM CaCl2, 10 mM lactose, 10 mM Beta-Cyclodextrin