CUTANA? pAG-MNase is the key reagent for performing Chromatin Immunocleavage (ChIC) [1] and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) [2,3]. As a fusion of Proteins A and G to Micrococcal Nuclease, CUTANA pAG-MNase is compatible with target antibodies from various host species and is highly purified to remove contaminating E. coli DNA, which can complicate analysis at low cell numbers. This enzyme enables efficient mapping of chromatin features in ChIC/CUT&RUN, allowing for significant improvements in signal to noise and sequencing depth compared to ChIP-seq.
反應(yīng)種屬:
宿主來源:
S. aureus
實(shí)驗(yàn)應(yīng)用:
靶標(biāo)/特異性:
同種型:
推薦稀釋度:
免疫原:
免疫原種屬:
克隆性:
克隆號:
純化方式:
偶聯(lián):
產(chǎn)品濃度:
表達(dá)系統(tǒng):
E. coli
陽性對照:
保存溫度:
Stable for one year at -20°C from date of receipt
運(yùn)輸溫度:
DO NOT FREEZE!! Frozen cold packs.
細(xì)胞定位:
預(yù)期分子量:
產(chǎn)品形式:
10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol