Epicypher新品 | 核酸提取緩沖液
CUTANA? 細(xì)胞核提取緩沖液是CUT&RUN和CUT&Tag實(shí)驗(yàn)中，用于從培養(yǎng)的細(xì)胞和組織中提取細(xì)胞核的必需試劑。這種緩沖液可與多種真核細(xì)胞和組織兼容。以下是使用該緩沖液成功進(jìn)行細(xì)胞核分離的細(xì)胞和組織類(lèi)型：
mouse NIH3T3 fibroblast cells | human A549 non-small cell lung cancer (NSCLC) cells |
human K562 leukemia cells | human NCI-H1299 non-small cell lung cancer (NSCLS cells) |
human bone marrow derived macrophages | human TIG-1 fetal lung cells |
human monocyte derived macrophages | human LoVo colorectal cancer cells |
human MV-4-11 macrophage cells | human LNCaP prostate carcinoma cells |
human SUM149 triple negative breast cancer (TNBC) cells | human renal primary cells |
human GM24385 B-lymphocyte (aka HG002) cells | human peripheral blood mononuclear cells (PBMCs) |
human MCF7 breast cancer cells | human intestinal tissue |
human MDA-MB-231 breast cancer cells | human HEPM embryonic cells |
human SK-MEL-2 melanoma cells |
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只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA? 細(xì)胞核提取緩沖液針對(duì)CUT&RUN和CUT&Tag實(shí)驗(yàn)流程進(jìn)行了優(yōu)化，從而獲得高質(zhì)量的細(xì)胞核，助力您的基因組圖譜分析。
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試劑盒組成
組分 | 貨號(hào) |
CUTANA??Pre-Nuclei Extraction Buffer? ? ?? | 21-1026a? ? ? |
1 M Spermidine | 21-1026b |
保存條件
將Pre-Nuclei Extraction Buffer 4oC儲(chǔ)存, 1 M亞精胺-20oC儲(chǔ)存。
自收到之日起可穩(wěn)定儲(chǔ)存6個(gè)月。
驗(yàn)證數(shù)據(jù)
Figure 1: Nuclei Extraction
Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA? Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA? Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.
訂購(gòu)詳情
貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 |
21-1026 | CUTANA? Nuclei Extraction Buffer | 100 Reactions |
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