來(lái)源于Escherichia coli（E.coli）的片段DNA可以用作核酸酶靶向切割和釋放（CUT&RUN）的實(shí)驗(yàn)標(biāo)準(zhǔn)化的spike-in對(duì)照。產(chǎn)品CUTANA? E. coli Spike-in DNA含有足夠的材料，可用于100-200個(gè)CUT&RUN樣本（高豐度和低豐度靶標(biāo)的范圍）。

產(chǎn)品詳情

保存溫度:?Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

運(yùn)輸溫度:?Room temp. Can go on frozen cold packs or dry ice.

產(chǎn)品形式:?100 ng lyophilized DNA. *NOTE: May not be visible.

驗(yàn)證數(shù)據(jù)

Figure 1: DNA gel data E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E.? coli cells (500 ng). Lane 2: Digested and purified CUTANA? E. coli Spike-in? DNA (500 ng) resolved via 2% E-Gel? EX Agarose Gel. Migration positions? of DNA molecular weight markers are indicated.

Figure 2: CUT&RUN sequencing data CUTANA? E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched? for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher? 13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads? aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE:? Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 -? 5%) of total sequencing reads.

訂購(gòu)詳情

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