EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher推出的CUT&RUN級別的BRD4 Antibody符合EpiCypher的“CUTANA Compatible”標準，可用于核酸酶靶向切割和釋放（CUT&RUN）和/或靶向剪切及轉(zhuǎn)座酶技術(shù)（CUT&Tag）的基因組定位。BRD4抗體主要在轉(zhuǎn)錄起始位點的側(cè)翼產(chǎn)生CUT&RUN峰(Fig. 1)。正如文獻所報道，BRD4峰與BRG1/SMARCA4峰有很大程度的重疊(Fig. 2)。

產(chǎn)品詳情

反應(yīng)種屬:?Human, Mouse

宿主來源:?Rabbit

實驗應(yīng)用:?CUT&RUN, IHC, IP, WB

免疫原:?Between amino acids 1312 and 1362

克隆性:?Polyclonal

保存溫度:?Stable for 1 year at 4°C from date of receipt

運輸溫度:?DO NOT FREEZE!! Frozen cold packs.

產(chǎn)品形式:?Antigen affinity-purified antibody in Tris-citrate/phosphate buffer pH 7-8, 0.09% sodium azide

驗證數(shù)據(jù)

Figure 1: BRD4 peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show BRD4 peaks relative to IgG negative control antibody and H3K4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. All rows were aligned relative to H3K4me3 antibody.

Figure 2: BRD4 CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci show overlap of BRD4 and BRG1 peaks.

Figure 3: Immunohistochemistry data

FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using BRD4 antibody at a dilution of 1:5,000.

Figure 4: Immunoprecipitation data

EpiCypher BRD4 antibody (6 μg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HeLa cells. A negative control IgG antibody and positive control antibody targeting BRD4 (Bethyl Laboratories) were also used to demonstrate the specificity of the IP. For blotting immunoprecipitates, Bethyl Laboratories BRD4 antibody (A700-004) was used at a dilution of 1:1,000.

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