關于BrdU
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU)，中文名為5-溴脫氧尿嘧啶核苷，是一種合成的核苷，胸腺嘧啶(thymidine)的類似物。BrdU普遍使用于檢測活組織中的增殖細胞，對研究細胞動力學有重要意義。
BrdU可被正在復制的細胞(S期)所攝取，整合至新合成的DNA鏈中，可取代胸腺嘧啶(thymidine)。然后可用BrdU特異性的抗體來檢測被攝入的BrdU，以此來反應細胞的增殖。DNA須經過變性(denaturation)才可與抗體結合，通常會用酸或熱來處理細胞。
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工欲善其事，必先利其器，BrdU檢測工具，您了解多少？
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BrdU細胞增殖ELISA檢測試劑盒
Exalpha公司研發(fā)的Frontier? BrdU 細胞增殖檢測分析試劑盒，是一種完全適用于高通量應用、非同位素標記法的ELISA檢測試劑盒(可提供顯色法和化學發(fā)光法)，可檢測摻入到貼壁細胞及非貼壁細胞新合成DNA鏈中的BrdU。
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試劑盒檢測原理
主要以細胞層作為固相載體, 將BrdU攝入微孔板中培養(yǎng)的細胞。在細胞培養(yǎng)的最后2-14小時，把BrdU加入微孔板中，BrdU將被摻入到分裂細胞的DNA中。為確?？贵w與BrdU有效結合，需要將細胞固定并做破膜處理，對DNA進行變性。所有操作僅需通過一步固定劑(Fixing solution)處理即可。將BrdUd單克隆抗體加入到板孔中，孵育1小時，未結合的抗體通過洗滌步驟去除，然后加入HRP標記的羊抗小鼠二抗，最后加入HRP底物顯色。
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| ?名稱 | ?貨號 | ?規(guī)格 | ?品牌 |
?Frontier? Bromodeoxyuridine (BrdU)
?Cell Proliferation ELISA Kit(比色法) | ?X1327K1? | ?200 tests | ?Exalpha |
?Frontier? Bromodeoxyuridine (BrdU)
?Cell Proliferation ELISA Kit(比色法) | ?X1327K2? | ?1000 tests | ?Exalpha |
?Frontier? Bromodeoxyuridine (BrdU)
?Cell Proliferation ELISA Kit(比色法) | ?X1327K3 | ?5000 tests | ?Exalpha |
?Frontier? Chemiluminescent
?Bromodeoxyuridine (BrdU) Cell
?Proliferation ELISA Kit（化學發(fā)光法） | ?X1623K1 | ?200 tests | ?Exalpha |
?Frontier? Chemiluminescent
?Bromodeoxyuridine (BrdU) Cell
?Proliferation ELISA Kit（化學發(fā)光法） | ?X1623K2 | ?1000 tests | ?Exalpha |
?Frontier? Chemiluminescent
?Bromodeoxyuridine (BrdU) Cell
?Proliferation ELISA Kit（化學發(fā)光法） | ?X1623K3 | ?5000 tests | ?Exalpha |
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BrdU免疫組化檢測試劑盒
BrdU被摻入到分裂細胞的DNA中，然后加生物素化的BrdU特異性抗體孵育，再通過HRP-鏈親和素結合物催化DAB底物顯色。該試劑盒可用對任何種屬的組織進行染色。
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| ?名稱 | ?貨號 | ?規(guī)格 | ?品牌 |
| ?Frontier? Bromodeoxyuridine (BrdU) Immunohistochemistry Kit | ?X1545K | ?50 slides | ?Exalpha |
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BrdU流式染色試劑盒
| ?名稱 | ?應用 | ?貨號 | ?品牌 |
| ?BrdU Staining Buffer Set | ?FC | ?00-5525 | ?eBioscience |
| ?BrdU Staining Kit for Flow Cytometry APC | ?FC | ?8817-6600 | ?eBioscience |
| ?BrdU Staining Kit for Flow Cytometry eFluor? 450 | ?FC | ?8848-6600 | ?eBioscience |
| ?BrdU Staining Kit for Flow Cytometry FITC | ?FC | ?8811-6600 | ?eBioscience |
| ?BrdU Staining Kit for Flow Cytometry PE | ?FC | ?8812-6600 | ?eBioscience |
| ?BrdU Staining Kit for Flow Cytometry PerCP-eFluor? 710 | ?FC | ?8846-6600 | ?eBioscience |
BrdU抗體
| 名稱 | 貨號 | 規(guī)格 | 品牌 |
| Sheep anti BrdU Polyclonal Antibody | A205P? | 250 μg | Exalpha |
| Mouse Anti-Bromodeoxyuridine Monoclonal Antibody | X1028 | 100 μg | Exalpha |
| Mouse Anti-Bromodeoxyuridine Monoclonal Antibody(FITC conjugate) | X1543M | 100 μg | Exalpha |
| RAT ANTI BrdU | MCA2060 | 0.2 mg | AbD Serotec |
| RAT ANTI BrdU:Alexa Fluor? 488 | MCA2060A488 | 100tests | AbD Serotec |
| RAT ANTI BrdU:Biotin | MCA2060B | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:FITC | MCA2060F | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:FITC | MCA2060FT | 0.5 ml | AbD Serotec |
| RAT ANTI BrdU | MCA2060GA | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:HRP | MCA2060P | 0.5 mg | AbD Serotec |
| RAT ANTI BrdU:HRP | MCA2060PA | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU | MCA2060T | 20 μg | AbD Serotec |
| MOUSE ANTI BrdU | MCA2483 | 0.2 mg | AbD Serotec |
| MOUSE ANTI BrdU:FITC | MCA2483FA | 50 μg | AbD Serotec |
| MOUSE ANTI BrdU | MCA2483T | 20 μg | AbD Serotec |
| RAT ANTI BrdU | OBT0030 | 0.5 mg | AbD Serotec |
| RAT ANTI BrdU | OBT0030CX | 0.25 ml | AbD Serotec |
| RAT ANTI BrdU:FITC | OBT0030F | 1 ml | AbD Serotec |
| RAT ANTI BrdU | OBT0030G | 0.25 mg | AbD Serotec |
| RAT ANTI BrdU | OBT0030S | 5 ml | AbD Serotec |
| BrdU [MoBu-1] antibody (Biotin) | GTX10362 | 100μg | GeneTex |
| BrdU antibody [BU6-4] | GTX12219 | 50μg | GeneTex |
| BrdU antibody | GTX128091 | 100μl | GeneTex |
| BrdU antibody [BU1/75 (ICR1)] | GTX26326 | 100μg | GeneTex |
| BrdU antibody [BU1/75 (ICR1)] (FITC) | GTX27384 | 250μl | GeneTex |
| BrdU antibody [B33.1] | GTX27781 | 500μl | GeneTex |
| BrdU antibody [B684M] | GTX44314 | 100μg | GeneTex |
| BrdU antibody [IIB5] (FITC) | GTX50089 | 500μl | GeneTex |
| BrdU, mAb (2B1) | ADI-MSA-200-E | 1*100ug | ENZO |
| 5-Bromodeoxyuridine, mAb (PRB-1) (biotin conjugate)? | ALX-804-197B-T060 | 60 tests? | ENZO |
| 5-Bromodeoxyuridine, mAb (PRB-1) (fluorescein conjugate)? | ALX-804-197L-T060 | 60 tests? | ENZO |
| Anti-BrdU APC | 17-5071 | 25/100tests | eBioscience |
| Anti-BrdU eFluor? 450 | 48-5071 | 25/100tests | eBioscience |
| Anti-BrdU FITC | 11-5071 | 25/100tests | eBioscience |
| Anti-BrdU PE | 12-5071 | 25/100tests | eBioscience |
| Anti-BrdU PerCP-eFluor? 710 | 46-5071 | 25/100tests | eBioscience |
| Anti-BrdU Purified | 14-5071 | 25/100ug | eBioscience |
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