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細(xì)胞凋亡(Apoptosis)檢測(cè)試劑盒

2021-05-17
瀏覽次數(shù): 124
細(xì)胞膜分析-PS外翻
● PS(磷脂酰絲氨酸)正常暴露在胞膜的內(nèi)表面,凋亡時(shí),PS外翻向細(xì)胞外表面。重組蛋白Annexin-V可與PS有高度親和力,又能與熒光染料結(jié)合。
●?PI(propidium iodide,碘化丙啶)是一種核酸染料,它不能透過完整的細(xì)胞膜,但對(duì)于凋亡中晚期的細(xì)胞和死細(xì)胞,PI 能夠透過細(xì)胞膜而使細(xì)胞核紅染。
●?7-AAD(7-amino-actinomycin ,D7-氨基放線菌素D)是一種核酸染料,它不能通過正常質(zhì)膜,隨著細(xì)胞凋亡、細(xì)胞死亡過程,質(zhì)膜對(duì)7-AAD的通透性逐漸增加,結(jié)合凋亡/死亡細(xì)胞中的DNA,在合適波長(zhǎng)激發(fā)光的激發(fā)下可發(fā)出明亮的紅色熒光,通過7-AAD標(biāo)記DNA的強(qiáng)弱,將細(xì)胞分為三群:7-AAD強(qiáng)為死亡細(xì)胞,7-AAD弱是凋亡細(xì)胞,7-AAD-為正?;盍?xì)胞。7-AAD同PI有著相似的熒光特性,但其發(fā)射波譜較PI窄,對(duì)其他檢測(cè)通道的干擾更小,在多色熒光分析中是PI的最佳替代品,可與Annexin V-EGFP/FITC/PE聯(lián)合使用。
細(xì)胞凋亡(Apoptosis)檢測(cè)試劑盒



細(xì)胞凋亡(Apoptosis)檢測(cè)試劑盒?
?供貨商
?產(chǎn)品名稱
?產(chǎn)品編號(hào)
?規(guī)格
?組分

?eBioscience
?Annexin V-FITC ? ?
?Apoptosis Detection Kit
?BMS500FI/100
?100 tests
?Annexin V-FITC, protein Binding Buffer (4x),
?Propidium Iodide (20 ug/mL)

?eBioscience
?BMS500FI/20
?20 tests
?eBioscience?
?BMS500FI/300
?300 tests
?eBioscience
?Annexin V-Biotin
?Apoptosis Detection Kit
?BMS500BT/100
?100 tests
?Annexin V-Biotin protein, Binding Buffer (4x), ?
?Propidium Iodide (20 ug/mL)

?eBioscience
?BMS500BT/20
?20 tests
?eBioscience
?Propidium Iodide 1.5 mL
?BMS500PI
?1.5 mL


?eBioscience
?anti-human Annexin
?V/FITC 100 tests
?BMS147FI
?100 tests


?eBioscience
?Binding Buffer for
?Annexin V(4x)
?BMS500BB*
?50 mL
?Resuspend cells used in Apoptosis
?detection Kit in Binding Buffer (1x).

?eBioscience
?Annexin V Apoptosis
?Detection Kit FITC
?88-8005-74
?200 tests
?10X Binding Buffer (cat. 00-0055),Annexin V ?
?FITC (cat. 11-8005),Propidium Iodide
?Staining Solution (cat. 00-6990)

?eBioscience
?88-8005-72
?50 tests
?eBioscience
?Annexin V Apoptosis
?Detection Kit eFluor?
?450
?88-8006-72
?50 tests
?10X Binding Buffer (cat. 00-0055),Annexin V
?eFluor? 450 (cat. 48-8006),7-AAD Viability
?Staining Solution (cat. 00-6993)
?可用于GFP轉(zhuǎn)染
?的細(xì)胞
?eBioscience
?88-8006-74
?200 tests
?eBioscience
?Annexin V Apoptosis
?Detection Kit APC
?88-8007-72
?50 tests
?10X Binding Buffer (cat. 00-0055),Annexin V
?APC (cat. 17-8007),Propidium Iodide
?Staining Solution (cat. 00-6990)
?可用于GFP轉(zhuǎn)染
?的細(xì)胞
?eBioscience
?88-8007-74
?200 tests
?eBioscience
?Annexin V Apoptosis
?Detection Kit PerCP-
?eFluor??710
?88-8008-72
?50 tests
?10X Binding Buffer (cat. 00-0055),Annexin V
?PerCP-eFluor??710 (cat. 46-8008),不能搭 ?
?配PI和7-AAD一起使用。未提供Fixable ?
?Viability Dye eFluor? 660 (cat. 65-0864)和
?Fixable Viability Dye eFluor? 780 (cat. 65-
?0865)
?沒有組分C,核
?酸染料
?eBioscience
?88-8008-74
?200 tests
?eBioscience
?Annexin V Apoptosis
?Detection Kit PE
?88-8102-72
?50 tests
?10X Binding Buffer (cat. 00-0055),? Annexin V
?PE (cat. 12-8102)7-AAD Viability Staining
?Solution (cat. 00-6993)
?可用于GFP轉(zhuǎn)染
?的細(xì)胞
?eBioscience
?88-8102-74
?200 tests
?eBioscience
?Annexin V Apoptosis
?Detection Kit PE-Cy7
?88-8103-72
?50 tests
?10X Binding Buffer (cat. 00-0055),? Annexin V
?PE-Cy7 (cat. 25-8103)
?注:不能和PI/7AAD一起搭配使用,可以與
?Fixable Viability Dye eFluor??660 (cat. 65-
?0864) 或者Fixable Viability Dye eFluor??780
?(cat. 65-0865) 一起染色。
?沒有組分C,核
?酸染料
?eBioscience
?88-8103-74
?200 tests
?eBioscience
?Propidium Iodide
?Staining Solution
?00-6990-50
?2 ml
?即用型,發(fā)射光(Blue、488 nm),發(fā)射光
?617nm

?eBioscience
?7-AAD Viability Staining
?Solution
?00-6993-50
?2 ml
?即用型,發(fā)射光(Blue、488 nm),發(fā)射光
?617nm

*PE-Cy7和PerCP-eFlour 710與細(xì)胞活性染料PI、7-AAD無法同時(shí)檢測(cè),所以試劑盒中沒有組分C,可以另外搭配Fixable Viability Dye eFluor 660(Cat#65-0864)或Fixable Viability Dye eFluor 780(Cat#65-0865)來進(jìn)行死活細(xì)胞的區(qū)分。


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