可固定的死活細(xì)胞鑒定染料( Fixable Viability Dye )可以不可逆地與死細(xì)胞結(jié)合,結(jié)合的死細(xì)胞可進(jìn)行清洗、固定、破膜等步驟以及冷凍保存,也不會(huì)對(duì)其熒光強(qiáng)度有影響。Fixable Viability Dye 可滲透到受損/壞死細(xì)胞的膜內(nèi),并與胞內(nèi)和膜上的自由氨基結(jié)合,形成強(qiáng)烈的熒光染色。而在活細(xì)胞,F(xiàn)ixable Viability Dye只能結(jié)合膜表面的自由氨基,F(xiàn)ixable?Viability Dye結(jié)合的死細(xì)胞的熒光強(qiáng)于活細(xì)胞50倍,從而區(qū)分死活細(xì)胞。與PI和7-AAD比,更具有競(jìng)爭(zhēng)優(yōu)勢(shì)。
?供貨商 | ?產(chǎn)品名稱(chēng) | ?產(chǎn)品編號(hào) | ?規(guī)格 | ?Excitation Laser | ?Peak Emission (nm) |
?eBioscience | ?Fixable Viability Dye eFluor450 | ?65-0863-14 | ?100 tests | ?Violet (405nm) | ?450 |
?65-0863-18 | ?500 tests(5×100) |
?eBioscience | ?Fixable Viability Dye eFluor660 | ?65-0864-14 | ?100 tests | ?Red (633 nm) | ?660 |
?65-0864-18 | ?500 tests(5×100) |
?eBioscience | ?Fixable Viability Dye eFluor780 | ?65-0865-14 | ?100 tests | ?Red (633 nm) | ?780 |
?65-0865-18 | ?500 tests(5×100) |
?eBioscience | ?Fixable Viability Dye eFluor? 506 | ?65-0866-14 | ?100 tests | ?Violet (405nm) | ?506(510/50 band pass filter )
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