DNA Damage (8-OHdG) ELISA Kit別名有8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit，下面是對這款產(chǎn)品的詳細(xì)介紹，如對本產(chǎn)品有不清楚的地方，歡迎咨詢Stressmarq中國代理商欣博盛生物。
研究領(lǐng)域：Cancer, Cell Signaling, DNA Damage and Repair, DNA/RNA, Epigenetics and Nuclear Signaling, Oxidation, Oxidative Stress, Post-translational Modifications
科學(xué)背景：8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
-產(chǎn)品信息
產(chǎn)品名稱：DNA Damage (8-OHdG) ELISA Kit
產(chǎn)品貨號(hào)：SKT-120
描述：Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
種屬反應(yīng)：Species Independent
Platform：Microplate
樣品類型：Cell lysates, Plasma, Sample matrices, Urine
檢測方法：Colorimetric Assay
檢測類型：Competitive ELISA (Enzyme-linked Immunosorbent Assay)
用途：ELISA Kit for 8-OHdG detection in samples.
靈敏度：0.59 ng/mL
分析范圍：0.94 - 60 ng/mL
精密度：Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be<5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
孵育時(shí)間：1 hour
樣本數(shù)目：39 samples in duplicate
應(yīng)用領(lǐng)域：Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
-產(chǎn)品屬性
儲(chǔ)藏溫度：4oC and -20oC
運(yùn)輸溫度：Blue Ice
產(chǎn)品類型：ELISAKits
概述：1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 μL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 μL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 μL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 μL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
文獻(xiàn)參考：
1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120.
2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34.
3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids.
4. Lin H., et al. (2004) Biochem J. 380: 541-548.
5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666.
6. Lee J., et al. (2005) Hypertension 45: 986-990.
7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152.
8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75.
9. Kuo H., et al. (2007) Mutat Res. 631:62-68.
10. Shen J., et al. (2007) Cancer 109: 574-580.
11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636.
12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110.
13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236.
14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
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