線粒體研究(Mitochondria?Research)
線粒體位于細(xì)胞核外,?既能產(chǎn)生能量又?jǐn)y帶遺傳信息,其結(jié)構(gòu)的特殊性決定其損傷及修復(fù)的方式與核DNA有所不同。檢測(cè)線粒體呼吸鏈功能對(duì)于診斷線粒體疾病是必要的。Cayman公司開發(fā)了線粒體復(fù)合體活性檢測(cè)試劑盒以及氧消耗率檢測(cè)試劑盒。
| ?產(chǎn)品名稱 | ?編號(hào) | ?規(guī)格 |
| ?MitoCheck?Complex?I?Activity?Assay?Kit | ?700930 | ?96?wells |
| ?MitoCheck?Complex?II?Activity?Assay?Kit | ?700940 | ?96?wells |
| ?MitoCheck?Complex?II/III?Activity?Assay?Kit | ?700950 | ?96?wells |
| ?Oxygen?Consumption?Rate?Assay?Kit(MitoXpress-Xtra?HS?Method) | ?600800 | ?96?wells |
| ?Oxygen?Consumption/MitoMembrane?Potential?Dual?Assay?Kit | ?600880 | ?96?wells |
轉(zhuǎn)錄因子分析?(Transcription?Factor?Assays)
Cayman研發(fā)的轉(zhuǎn)錄因子分析技術(shù)，采用非放射性、高靈敏度的方法，檢測(cè)細(xì)胞核提取物中轉(zhuǎn)錄因子與DNA的特異性結(jié)合活性。該技術(shù)取代傳統(tǒng)繁雜的EMSA方法，用96孔板形式來分析轉(zhuǎn)錄因子與DNA的結(jié)合。主要技術(shù)原理為，首先將特異性dsDNA序列(含應(yīng)答元件)包被固定于96孔板中，再將含轉(zhuǎn)錄因子的核提取物加入板孔中孵育，轉(zhuǎn)錄因子與包被的DNA序列特異性結(jié)合，然后用轉(zhuǎn)錄因子特異性的一抗孵育，最后加入相應(yīng)的二抗-HRP結(jié)合物，顯色并于450nm讀數(shù)。
| ?產(chǎn)品名稱 | ?編號(hào) | ?規(guī)格 |
| ?ATF2(Phospho-Thr69,71)?Transcription?Factor?Assay?Kit | ?600130 | 96?wells |
| ?ChREBP?Cell-Based?Translocation?Assay?Kit? | ?10010060 | ?96?wells |
| ?ChREBP?Transcription?Factor?Assay?Kit | ?10006909 | ?96?wells |
| ?CREB(Phospho-Ser133)Transcription?Factor?Assay?Kit | ?10009846 | ?96?wells |
| ?HIF-1alpha?Transcription?Factor?Assay?Kit | ?10006910 | ?96?wells |
| ?NF-kB(human?p50)?Transcription?Factor?Assay?Kit | ?10006912 | ?96?wells |
| ?NF-kB(p65)?Transcription?Factor?Assay?Kit | ?10007889 | ?96?wells |
| ?NF-kB(p50/p65)?Combo?Transcription?Factor?Assay?Kit | ?10011223 | ?96?wells |
| ?Nrf2?Transcription?Factor?Assay?Kit | ?600590 | ?96?wells |
| ?p53?Cell-Based?Activation/Translocation?Assay?Kit | ?600008 | ?96?wells |
| ?p53?Designer?Transcription?Factor?Assay?Kit | ?600030 | ?96?wells |
| ?p53?total?and?p53(Phospho-Ser392)?Dual?Staining?Assay?Kit | ?600060 | ?96?wells |
| ?p53?Transcription?Factor?Assay?Kit | ?600020 | ?96?wells |
| ?PPARα?Transcription?Factor?Assay?Kit | ?10006915 | ?96?wells |
| ?PPARδ?Transcription?Factor?Assay?Kit | ?10006914 | ?96?wells |
| ?PPARγ?Transcription?Factor?Assay?Kit | ?10006855 | ?96?wells |
| ?PPARα,δ,γ?Complete?Transcription?Factor?Assay?Kit | ?10008878 | ?96?wells |
| ?PPARγ?Ligand?Screening?Assay?Kit | ?10007685 | ?96?wells |
| ?SREBP-1?Transcription?Factor?Assay?Kit | ?10010854 | ?96?wells |
| ?SREBP-2?Cell-Based?Translocation?Assay?Kit | ?10009239 | ?96?wells |
| ?SREBP-2?Transcription?Factor?Assay?Kit | ?10007819 | ?96?wells |
| ?Nuclear?Extraction?Kit | ?10009277 | ?1?ea |