Enzo Life Sciences的ROS-ID??NO Detection kit以紅色熒光的NO檢測試劑為主要成分，可通過熒光顯微鏡直接實時監(jiān)測活細胞中NO的產(chǎn)生。這種非熒光的、可滲透細胞的NO檢測染料在O2存在的情況下能夠與NO發(fā)生反應(yīng)，具有高特異性、靈敏性和準確性，產(chǎn)生不溶于水的紅色熒光產(chǎn)物。這種染料對過氧亞硝酸鹽沒有反應(yīng)性，因此可以區(qū)分過氧亞硝酸鹽和一氧化氮。
ROS-ID??NO Detection kit中的熒光探針對活性氯或溴不敏感，因此不用于檢測這些分析物。染色后，該染料產(chǎn)生的熒光產(chǎn)物可通過熒光顯微鏡進行觀察。當需要同時檢測額外的熒光信號（綠色或橙色）時，建議使用650/670 nm的組合。ROS-ID??NO Detection kit包含足夠的試劑，可對活細胞（貼壁或懸浮）進行至少200次檢測。
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產(chǎn)品特色
通過熒光顯微鏡直接實時監(jiān)測活體細胞中NO的產(chǎn)生
高特異性、靈敏性和準確性，產(chǎn)生不溶于水的紅色熒光產(chǎn)物
可區(qū)分過氧亞硝酸鹽和一氧化氮
試劑盒內(nèi)包含一氧化氮誘導劑和清除劑
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產(chǎn)品信息
產(chǎn)品貨號 | ENZ-51013-200 |
產(chǎn)品名稱 | ROS-ID??NO Detection kit |
規(guī)格 | 1*1Kit |
操作 | Protect from light. Avoid freeze/thaw cycles. |
短期保存 | -20°C |
長期保存 | -80°C |
試劑盒組分 | NO Detection Reagent (Red), 60 μl NO Inducer (L-Arginine), 100 μl NO Scavenger (c-PTIO), 400 nmoles 10X Wash Buffer, 15 ml |
應(yīng)用 | Fluorescence microscopy, Fluorescent detection |
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部分產(chǎn)品引用文獻
1.?Human M1 macrophages express unique innate immune response genes after mycobacterial infection to defend against tuberculosis: A. Karshan, et al.; Commun. Biol. 5, 480 (2022)
2.?Noncanonical Role of Telomerase in Regulation of Microvascular Redox Environment With Implications for Coronary Artery Disease: K.A. Aissa, et al.; Function 3, zqac043 (2022)
3.?S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)
4.?High-content analysis monitoring intracellular trafficking and replication of Mycobacterium tuberculosis inside host cells: N. Debousere, et al.; Methods Mol. Biol. 2314, 649 (2021)
5.?Hyperhomocysteinemia and Low Folate and Vitamin B12 Are Associated with Vascular Dysfunction and Impaired Nitric Oxide Sensitivity in Morbidly Obese Patients: M. Haloul, et al.; Nutrients 12, 2014 (2020)
6.?Stimulation of TRPA1 attenuates ischemia-induced cardiomyocyte cell death through an eNOS-mediated mechanism: S.R. Andrei, et al.; Channels (Austin) 13, 192 (2019)
7.?Anti-inflammatory effects of olive-derived hydroxytyrosol on lipopolysaccharide-induced inflammation in RAW264.7 cells: Y. Yonezawa, et al.; J. Vet. Med. Sci. 80, 1801 (2018)
8.?Oligodendrocyte RasG12V expressed in its endogenous locus disrupts myelin structure through increased MAPK, nitric oxide, and notch signaling: H.E. Titus, et al.; Glia 65, 1990 (2017)
9.?Improved arterial flow-mediated dilation after exertion involves hydrogen peroxide in overweight and obese adults following aerobic exercise training: A.T. Robinson, et al.; J. Hypertens. 34, 1309 (2016)
10.?STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection: C.J. Queval, et al.; Sci. Rep. 6, 29297 (2016)
11.?Flavonoids activate endothelial nitric oxide synthase by altering their phosphorylation via mitogen-activated protein kinase pathways in glucose-induced endothelial cells: C.E. Kim, et al.; J. Funct. Foods 17, 676 (2015)
12.?Ginsenoside Rg3 regulates S-Nitrosylation of the NLRP3 inflammasome via suppression of iNOS: S. J. Yoon, et al.; Biochem. Biophys. Res. Commun. 463, 1184 (2015)
13.?Propofol restores TRPV1 sensitivity via a TRPA1-, nitric oxide synthase-dependent activation of PKCε: P. Sinharoy, et al.; Pharmacol. Res. Perspect. 3, e00153 (2015)
14.?Reduced flow-and acetylcholine-induced dilations in visceral compared to subcutaneous adipose arterioles in human morbid obesity: I. Grizelj, et al.; Microcirculation 22, 44 (2015)
15.?Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia: Y. Yuan, et al.; BMC Neurosci. 15, 125 (2014)
16.?Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism: Z.G. Khalil, et al.; Mycology 5, 168 (2014)
17.?Cyclosporine attenuates arginine transport, in human endothelial cells, through modulation of cationic amino acid transporter-1: A. Grupper, et al.; Am. J. Nephrol. 37, 613 (2013)
18.?Identification and characterization of a functional mitochondrial angiotensin system: P.M. Abadir, et al.; PNAS 108, 14849 (2011)
19.?Loss of bcl-2 during the senescence exacerbates the impaired angiogenic functions in endothelial cells by deteriorating the mitochondrial redox state: M. Uraoka, et al.; Hypertension 58, 254 (2011)
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