Enzo Life Sciences的GFP-CERTIFIED??FLUOFORTE??Calcium assay kit提供了一種熒光分析方法，用于檢測廣泛生物靶標(biāo)的細(xì)胞內(nèi)鈣動(dòng)員情況。相對(duì)于其他商業(yè)化的染料，GFP-CERTIFIED??FLUOFORTE?染料是最亮和最靈敏的熒光鈣指示劑。該試劑盒為貼壁和非貼壁細(xì)胞系提供了更少步驟，更低變異性，更簡易的鈣檢測。此外，它既不需要洗滌步驟，也不需要添加外源性淬滅染料，避免了對(duì)受體-配體相互作用動(dòng)力學(xué)的干擾。
Enzo Life Sciences的GFP-CERTIFIED??FLUOFORTE??Calcium assay kit可用于細(xì)胞內(nèi)鈣離子的高通量檢測，鈣離子是重要的第二信使，常用于初步篩選中監(jiān)測G蛋白偶聯(lián)受體激活。
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產(chǎn)品特色
● 可監(jiān)測表達(dá)GFP細(xì)胞中的鈣離子動(dòng)員情況
●?最明亮、最靈敏的熒光Ca2+指示劑
●?獲得的 EC50和IC50值與使用鈣離子熒光探針 Rhod-4 AM和Fluo-4 AM 相當(dāng)
●?更大范圍的檢測窗口
●?使用方便，無需洗滌，無需淬滅染料
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實(shí)驗(yàn)示例
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圖2. U-2 OS細(xì)胞分別與（A）GFP-CERTIFIED??FLUOFORTE?AM染料和（B）Rhod-2 AM染料孵育。洗滌細(xì)胞并用終濃度為100nM的ATP處理，并立即成像。GFP-CERTIFIED??FLUOFORTE??AM染料比Rhod-2 AM表現(xiàn)出更亮的熒光強(qiáng)度。
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ENZ-51020-KP010/ ENZ-51020-KP100 |
產(chǎn)品名稱 | GFP-CERTIFIED??FLUOFORTE??Calcium assay kit |
規(guī)? ? ? ?格 | 10*96wells/100*96wells |
短期保存 | -20°C |
長期保存 | -80°C |
試劑盒組分 | Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED??FLUOFORTE??dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates
High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED??FLUOFORTE??dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates |
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部分產(chǎn)品引用文獻(xiàn)
1.?Perfluorooctane sulfonate and bisphenol A induce a similar level of mast cell activation via a common signaling pathway, Fyn-Lyn-Syk activation: S.J. Park, et al.; Food Chem. Toxicol. 156, 112478 (2021)
2.?horbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner: Y.Y. Chang, et al.; Nitric Oxide 109, 33 (2021)
3.?Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation: C. Tr?tschel, et al.; EMBO J. 39, e102723 (2020)
4.?Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium: J.N. Hansen, et al.; Elife 9, e57907 (2020)
5.?Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)
6.?Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)
7.?Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)
8.?Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)
9.?T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)
10.?Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)
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