睪酮(Testosterone)又稱睪丸素、睪丸酮、睪甾酮，是分泌到血液中的最重要的雄性激素之一，由孕烯醇酮合成，孕烯醇酮本身由膽固醇形成。睪酮由男性的睪丸或女性的卵巢分泌，腎上腺亦分泌少量睪酮，其具有維持肌肉強(qiáng)度及質(zhì)量、維持骨質(zhì)密度及強(qiáng)度、提神及提升體能等作用。在成人中，每天大約合成5毫克睪酮，并在血漿中循環(huán)，主要與蛋白質(zhì)結(jié)合，包括特異性激素結(jié)合球蛋白(SHBG)和非特異性蛋白質(zhì)如白蛋白。據(jù)報(bào)道，生物可利用的睪酮包括游離類固醇和白蛋白結(jié)合類固醇，它們約占總睪酮的35%。
ENZO?Testosterone ELISA kit是一種比色競(jìng)爭(zhēng)法酶免疫檢測(cè)試劑盒，3小時(shí)內(nèi)可以得到檢測(cè)結(jié)果。在405nm處讀取吸光度。
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●?高靈敏度，最低可檢測(cè)5.67pg/ml睪酮
●?高通量，3小時(shí)內(nèi)可得到檢測(cè)結(jié)果
●?批間一致性好，是縱向研究的理想選擇
●?超越半定量Western Blot分析的全定量檢測(cè)
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ADI-900-065/ ADI-901-065 |
產(chǎn)品名稱 | Testosterone ELISA kit（睪酮ELISA試劑盒） |
規(guī)格 | 96 wells/?5x96 wells |
靈敏度 | 5.67 pg/ml |
檢測(cè)范圍 | 7.81 - 2,000 pg/ml |
檢測(cè)時(shí)間 | 3 hours |
樣本種屬 | Species independent |
交叉反應(yīng) | Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and<0.001%: Dihydrotestosterone, Estriol, Aldosterone, Corticosterone, Cortisol, Cortisone, Estrone, Progesterone, Pregenolone |
樣本類型 | culture supernatants, plasma, serum, and saliva from any species. Not suitable for human serum and plasma. For those samples use product ADI-900-176. Cited sample types include tissue and feces |
保存溫度 | +4°C |
試劑盒組分 | GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, Steroid displacement reagent, pNpp Substrate, Stop solution |
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部分產(chǎn)品引用文獻(xiàn)
1. 3-Monochloropropane-1,2-diol causes spermatogenesis failure in male rats via Sertoli cell dysfunction but not testosterone reduction: H. Xing, et al.; Toxicol. Lett. (2022)?
2. Androgen conspires with the CD8+ T cell exhaustion program and contributes to sex bias in cancer: H. Kwon, et al.; Sci. Immunol. 7, eabq2630 (2022)
3. Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022)?
4. Exogenous testosterone administration decreases rates of ovarian follicle growth in domestic chickens: C.R. Dean, et al.; Gen. Comp. Endocrinol. 329, 114121 (2022)?
5. Non-invasive assessment of fecal glucocorticoid, progesterone, and androgen metabolites and microbiome in free-ranging southern white rhinoceros (Ceratotherium simum simum) in South Africa: K.H. Kothmann, et al.; Gen. Comp. Endocrinol. 329, 114099 (2022)
6. Purple Corn Extract Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis: H.J. Kim, et al.; J. Agric. Food Chem. 70, 5561 (2022)
7. Sex, Organ, and Breed Differences in the mRNA Expression of Drug Transporters in the Liver and Kidney of Pigs: M. Kojima, et al.; Biol. Pharm. Bull. 45, 508 (2022)?
8. Social dominance and reproduction result in increased integration of oxidative state in males of an African cichlid fish: R.J. Fialkowski, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 269, 111216 (2022)?
9. Androgen receptor phosphorylated at Ser815: The expression and function in the prostate and tumor-derived cells: K. Yokobori, et al.; Biochem. Pharmacol. 194, 114794 (2021)?
10. Androgen-Dependent Differences in the Amounts of CYP mRNAs in the Pig Kidney: M. Kojima, et al.; Biol. Pharm. Bull. 44, 1120 (2021)?
11. Early nighttime testosterone peaks are correlated with GnRH-induced testosterone in a diurnal songbird: T. Greives, et al.; Gen. Comp. Endocrinol. 312, 113861 (2021)?
12. Exercise Training Promotes Sex-Specific Adaptations in Mouse Inguinal White Adipose Tissue: P. Nigro, et al.; Diabetes 70, 1250 (2021)?
13. Impact of prenatal arsenic exposure on the testes and epididymides of prepubertal rats: A.C.F. Souza, et al.; Chem. Biol. Interact. 333, 109314 (2021), Application(s): ELISA using rat serum?
14. Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats: K.S. Lee, et al.; Food Sci. Biotechno. 30, 443 (2021), Application(s): Blood samples
15. Investigation of equine testis contribution to vitamin D bioactivation: M. Rouge, et al.; Domest. Anim. Endocrinol. 79, 106691 (2021), Application(s): ELISA using horse plasma
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