BioXcell熱銷產(chǎn)品--BP0273 ?InVivoPlus anti-mouse PD-1 (CD279)
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產(chǎn)品描述：
BioXcell InVivoPlus anti-mouse PD-1 29F.1A12單克隆抗體與小鼠PD-1（也稱為CD279）反應(yīng)。PD-1是一種50-55kDa的細(xì)胞表面受體，由Pdcd1基因編碼，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和髓細(xì)胞上瞬時(shí)表達(dá)，在成功消除抗原后PD-1的表達(dá)下降。此外，在B細(xì)胞前階段，Pdcd1 mRNA在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括ITIM（基于免疫受體酪氨酸的抑制基序），這表明PD-1負(fù)調(diào)控TCR信號(hào)。PD-1通過(guò)結(jié)合B7家族的成員PD-L1和PD-L2發(fā)出信號(hào)。在配體結(jié)合后，PD-1信號(hào)傳導(dǎo)抑制T細(xì)胞活化，導(dǎo)致增殖減少，細(xì)胞因子產(chǎn)生和T細(xì)胞死亡。此外，PD-1敲除動(dòng)物表現(xiàn)出擴(kuò)張型心肌病、脾腫大和外周耐受喪失，在小鼠的外周耐受性和預(yù)防自身免疫性疾病中發(fā)揮關(guān)鍵作用。誘導(dǎo)的PD-L1表達(dá)常見(jiàn)于許多腫瘤，包括鱗狀細(xì)胞癌、結(jié)腸癌和乳腺癌。PD-L1過(guò)度表達(dá)會(huì)導(dǎo)致腫瘤細(xì)胞對(duì)CD8T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑素瘤的小鼠模型中，可以通過(guò)用阻斷PD-L1與其受體PD-1之間相互作用的抗體治療來(lái)暫時(shí)抑制腫瘤生長(zhǎng)。由于這些原因，目前正在探索抗PD-1介導(dǎo)的免疫療法作為癌癥治療。與RMP1-14和J43抗體一樣，BioXcell InVivoPlus anti-mouse PD-1 29F.1A12抗體在體內(nèi)可阻斷PD-1與其配體的結(jié)合。
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產(chǎn)品詳情：
| 產(chǎn)品名稱 | InVivoPlus anti-mouse PD-1 (CD279) |
產(chǎn)品貨號(hào) | BP0273 |
產(chǎn)品規(guī)格 | 5/25/50/100mg |
反應(yīng)種屬 | Mouse |
克隆號(hào) | 29F.1A12 |
同種型 | Rat IgG2a |
免疫原 | Recombinant PD-1-Ig fusion protein |
實(shí)驗(yàn)應(yīng)用 | in vivo blocking of PD-1/PD-L signaling in vitro PD-1 neutralization Immunohistochemistry (frozen) Immunofluorescence Western blot Flow cytometry |
產(chǎn)品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
純度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
無(wú)菌處理 | 0.2 μm filtration |
純化方式 | Protein G |
RRID | AB_2687796 |
分子量 | 150 kDa |
小鼠病原檢測(cè) | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存條件 | 抗體原液保存在4°C，不能冷凍保存。 |
推薦同型對(duì)照 | InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（貨號(hào)BP0089） |
推薦抗體稀釋液 | InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070） |
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為什么選擇InVivoPlus抗體？
InVivoPlus級(jí)別的產(chǎn)品內(nèi)毒素含量更低，經(jīng)過(guò)多種實(shí)驗(yàn)驗(yàn)證，更適合用于體內(nèi)實(shí)驗(yàn)研究
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?該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：
應(yīng)用 | 文章 |
體內(nèi)PD-1/PD-L信號(hào)阻斷 (in vivo blocking of PD-1/PD-L signaling) | 1.?Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757. 2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499. 3.?Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008. |
體內(nèi)PD-1/PD-L信號(hào)阻斷，流式細(xì)胞術(shù) (in vivo blocking of PD-1/PD-L signaling, Flow Cytometry) | 1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501. |
體外PD-1中和 （in vitro PD-1 neutralization） | 1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629. |
體內(nèi)PD-1/PD-L信號(hào)阻斷，體外PD-1中和 （in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization） | 1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603. |
流式細(xì)胞術(shù) （Flow Cytometry） | 1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225. |
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