克隆號(hào)UCHT1 (Leu-4)(T3)單克隆抗體與人CD3ε一種屬于免疫球蛋白超家族的20 kDa跨膜細(xì)胞表面蛋白反應(yīng)。CD3ε是結(jié)合形成TCR復(fù)合物的五個(gè)多肽鏈之一。CD3ε在T淋巴細(xì)胞NK-T細(xì)胞上表達(dá),并在不同程度上在發(fā)育中的胸腺細(xì)胞上表達(dá)。CD3在TCR信號(hào)T淋巴細(xì)胞活化和抗原識(shí)別中發(fā)揮作用。通過(guò)固定化UCHT1 (Leu-4)(T3)抗體交聯(lián)TCR通常用于體外激活T細(xì)胞。
(InVivoMAb anti-human CD3產(chǎn)品圖片)
產(chǎn)品詳情
產(chǎn)品貨號(hào) | BE0231 |
產(chǎn)品規(guī)格 | 1/5/25/50/100mg |
抗體亞型 | Mouse IgG1,?κ |
推薦同型對(duì)照 | InVivoMAb mouse IgG1 isotype control, unknown specificity(貨號(hào):BE0083) |
推薦抗體稀釋液 | InVivoPure? pH7.0 Dilution Buffer(貨號(hào):IP0065) |
免疫原 | Human CD3ε |
應(yīng)用 | in vivo T cell depletion in humanized mice ex vivo T cell inhibition for xenographs Flow cytometry |
產(chǎn)品形式 | PBS , pH 7.0 Contains no stabilizers or preservatives |
內(nèi)毒素水平 | <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 測(cè)定 |
純度 | >95% Determined by SDS-PAGE |
無(wú)菌處理 | 0.2 μM filtered |
生產(chǎn)形式 | 從組織培養(yǎng)上清液中純化得到。 |
純化形式 | Protein G |
RRID | AB_2687713 |
分子量大小 | 150 kDa |
保存條件 | 抗體原溶液應(yīng)保存在4°C條件下,不要冷凍保存。 |
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已發(fā)表參考文獻(xiàn):
in vivo T cell depletion in humanized mice | Wunderlich, M., et al. (2014). “OKT3 prevents xenogeneic GVHD and allows reliable xenograft initiation from unfractionated human hematopoietic tissues.” Blood 123(24): e134-144. |
in vivo T cell depletionn | Woo, J. H., et al. (2010). “Pharmacology of anti-CD3 diphtheria immunotoxin in CD3 positive T-cell lymphoma trials.” Methods Mol Biol 651: 157-175. |
Flow cytometry | Rossi, N. E., et al. (2008). “Differential antibody binding to the surface alphabetaTCR.CD3 complex of CD4+ and CD8+ T lymphocytes is conserved in mammals and associated with differential glycosylation.” Int Immunol 20(10): 1247-1258. |
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